Intended use of the testkit
The Borrelia SRE testkit is designed to detect antibodies against Borellia proteins. Borrelia proteins are attached to the solid phase. After washing the strips are incubated with the dog sera to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labelled anti-species conjugate is added to detect bound dog antibodies to Borrelia proteins. After incubation and rinsing the substrate is added and the optical density is measured at 620nm or 450nm..
Principle of the testkit
The test is based on the reaction of Borrelia proteins with a polyclonal dog antibodies. To this end Borrelia proteins have been coated to a 32-well microtiter plate.
The diluted dog serum/plasma sample is added to the wells of the coated plate. After washing the bound dog antibodies are detected by a HRPO conjugated anti species cconjugate. The color reaction in the wells is directly related to the concentration of Borrelia antibodies in the serum/plasma sample.