Rota Virus antigen EIA 96 well plate


A monoclonal antibody-mediated ELISA for the detection of Rotavirus in faeces samples (type A).

Article code: EIA AS1001-AG02


SKU: P0221 Category:


Intended use of the testkit

The Rota virus antigen ELISA test kit is designed to detected Rota virus infections in all kind of species (including humans).The principle of the test is based on the reaction of a monoclonal (mix) catching phase and a polyclonal detecting antibody which detected different conserved epitopes.

Principle of the testkit

The principle of the test is based on the reaction of Rota antigen with monoclonal anti-Rota antibodies. To this end these monoclonal antibodies are coated to a 96 well microtiter strip plate.

The feaces sample is added (diluted 1:1) to the wells of the coated plate. After incubation and washing, the bound Rota antigen is detected by a polyclonal anti-Rota antibody. After incubation and washing the bound polyclonal antibodies are detected by a HRPO conjugated anti-species IgG conjugate. Color reaction in the wells is directly related to the concentration of the Rota antigen in the feaces sample.

Interpretation of test results

A sample is considered positive when the measured extinction is higher than 3 times the OD value of the negative control (≥ 0,200). The OD value of the positive control must be higher or equal to 1,000 OD units.


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