Intended use of the testkit
The Influenza Virus type A ELISA is designed these viral proteins of IV. To this end monoclonal antibodies have been bound to the solid phase. The plates are incubated with the sample to be best tested. The plates are washed after incubation to remove unbound materials. A monoclonal antibody against influenza, conjugated with biotin, is added to detect bound Influenza Virus. The plates are washed after incubation to remove unbound materials. Conjugated streptavidin is added to detect bound biotin conjugate. After incubation and washing, the substrate is added and the optical density is measured at 450 nm.
Principle of the testkit
The test is based on the reaction of antibodies with NP proteins. To this end, monoclonal antibodies have been coated to a 96 well microtiter strip plate.
The sample is added (in a titration pure, 1:10, 1:30 and 1:90 diluted) to the wells of the coated plate. After washing, the bound influenza virus is detected by an anti-influenza biotin conjugate. After washing, the bound anti-Influenza conjugate is detected by streptavidin HRPO conjugate. Bound streptavidin conjugate is made visible by adding substrate/chromagen mix. The intensity of the color reaction in the wells is directly correlated to the concentration of Influenza virus in the sample.
Interpretation of test results
A sample is considered positive when the measured extinction is higher than 2 times the OD of the negative control. When a sample is negative > sample < 2x negative, it should be tested again within 2-4 weeks. It is advised to keep animals isolated during this time frame. The OD of the positive control must be ≥ 1,000.