Intended use of the testkit
The Influenza typa A virus (AIV NP) antistof ELISA is designed to detect antibodies against the proteins. To this end recombinant NP proteins are bound to the solid phase. After washing the plates are incubated with the bird sera to be tested. The plates are washed after incunation to remove unbound materials. An anti-species conjugate is adde to detect bound antibodies to AIV. After incubation and washing, the substrate is added and the optical density is measured at 450 nm.
Principle of the testkit
The principle of test is based on the reaction op NP proteins with avian antibodies. To this end, NP expression proteins have been coated to 96 well microtiter strip plate.
The serum sample is added (dilutted 1:100) to the wells of the coated plate. After washing, the bound avain antibodies are detected by an anti-species conjugate. Bound anti-species conjugate is made visible by adding substrate/chromagen mix. The intensity of the color reaction in the wells is propotrional to the concentration of anti-AIV antibodies in the serum sample.
Interpretation of test results
A sample is considered positive when the measured extinction is at least 3 times the OD value of the negative control.
When a sample read higher than the negative control but less than 3 times the negative control it should be tested again within 2-4 weeks. It is advised to keep animals isolated during this period.
The OD value of the positive control must be ≥ 0,500.