Intended use of the testkit
This diagnostic test is itended to identify antibodies against the antigen of BHV-1 virus, in serum and plasma samples.
Principle of the testkit
The test is based on the reaction of anti-IBR antibodies with BHV-1 antigen. To this end these BHV-1 antigens are coated to a 96-well microtiter strip-plate. The bovine serum or plasma sample is added to the wells of the coated plate.
After incubation, an anti-BHV-1 conjugate is added to complete for the BHV-1 antigen that is coated to the 96-well microtiter strip-plate. Bound conjugate is made visible by adding substrate/chromagen mix. Itensity of the color reaction in the wells is directly correlated to the concentration of anti-IBR antibodies in the serum, plasma or milk sample.
Interpretation of test results
A sample is considered positive when the ratio is ≤0,6.
A sample is considered negative when the ratio is ≥0,7
A sample should be retested when the ratio is between 0,6 and 0,7.
Ratio= Sample OD value / Negative control OD value