Intended use of the testkit
The feline Toxoplasma SRE testkit is designed to detect antibodies against Toxoplasma infections. After washing the strips are incubated with the cat sera to be tested. The strips are washed after incubation to remove unbound materials. A HPRO labeled anti-species conjugat is added and the optical density is measured at 620nm.
Principle of the testkit
The test is based on the reaction of toxoplasma proteins with polyclonal cat antibodies. To this end Toxoplasma proteins have been coated to a microtiter plate. The diluted cat serum/plasma sample is added to the wells of the coated plate. After washing the bound cat antibodies are detected by a HRPO conjugated anti-species conjugate.
The color reaction in the wells is directly related to the concentration of Toxoplasma antibodies in serum/plasma samples.