Intended use of the testkit
The FCoV Rapid ELISA testkit is designed to detect antibodies againnst FCoV proteins (mostly glycoproteins). FCoV proteins are attached to the solid phase. After washing, the strips are incubated with the cat sera, to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labelled anti-species conjugate is added to detect bound cat antibodies to FCoV proteins. After incubation and rinsing the substrate is added and the optical density is measured at 620 nm.
Principle of the testkit
The test is based on the reaction of FCoV proteins (mostly glycoprotein’s) with polyclonal cat antibodies. To this end purified FCoV proteins have been coated to a 32-well microtiter plate. The diluted cat serum sample is added to the wells of the coated plate. After washing the bound cat antibodies are detected by a HRPO conjugated anti-species conjugate.
The color reaction in the wells is directly related to the concentration of FCoV antibodies in the serum.