Equine Immunodeficiency Virus-p24 Antibody ELISA (96 wells)

 344,00

An ELISA test to detect antibodies against Equine Immunodeficiency Virus-p24 antigen (EIV) in serum or plasma samples.

SKU: EQ1002-AB01 Categories: ,

Description

Intended use of the test kit

The EIV p24 ELISA is designed to detect antibodies against these proteins. To this end recombinant p24 proteins are attached to the solid phase. After washing, the plates are incubated with the horse sera to be tested. The plates are washed after incubation to remove unbound materials. An anti-species conjugate is added to detect bound cat antibodies to EIV p24. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of p24 proteins with horse antibodies. To this end p24 expression proteins have been coated to a 96 well microtiter strip plate.

  • Qualitative
    The sample is added (diluted 1:50) to the wells of the coated plate.
  • Quantitative
    The sample also can be titrated using a 3-step dilution, starting with a dilution 1:30 (> 1:90 > 1:270 > 1:810).

After washing, the bound horse antibodies are detected by an anti-species conjugate.
Bound anti-species conjugate is made visible by adding substrate/chromagen mix. The intensity of the color reaction in the wells is directly correlated to the concentration of anti-EIV p24 antibodies in the serum sample.

Protocol

Equine Immunodeficiency Virus-p24 Antibody ELISA

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