Classical Swine Fever Antibody ELISA

 321,88 901,82

An ELISA testkit to detect antibodies against Classical Swine Fever Virus (CSFV) recombinant protein in serum or plasma samples.

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SKU: P1003-AB02 Categories: ,

Description

Intended use of the test kit

This diagnostic test is intended to identify CSFV antibodies, in serum and plasma samples.
Microtiter plates are coated with recombinant proteins, antibodies in the sera or plasma bind to these proteins. Bound antibodies are detected by use of anti-species conjugate.

Principle of the test kit

The test is based on the reaction of coated recombinant proteins within pig antibodies. To this end, recombinant proteins have been coated to a 96 well microtiter strip plate.

  • Qualitative
    The sample is added (diluted 1:100) to the wells of the coated plate.
  • Quantitative
    The sample also can be titrated using a 3-step dilution, starting with a dilution 1:100 (> 1:300 > 1:900 > 1:2700).

After washing, the bound pig’s antibodies are detected by an anti-Swine HRPO conjugate, which will bind to the bound Pig antibodies. Bound complex is made visible by adding substrate/chromogen mix.
The intensity of the color reaction in the wells is directly correlated to the concentration of anti-CSFV antibodies in the serum or plasma sample.

Protocols

CSFV Antibody ELISA

CSFV Antibody ELISA 5 plate

Additional information

Quantity

96, 480

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