Principle of the testkit
The progesterone testkit is based on monoclonal antibodies against a epitope of progesterone, which are coated to the solid phase. If the sample contains the progesterone hormone, the progesterone will bind to monoclonal antibody (solid phase). If the sample contains a low level of progesterone the conjugate will bind to the solid phase. If the sample contains a high level of progesterone the conjugate will not bind the solid phase. When the substrate is added color will develop at the samples with low levels of progesterone, if the sample contains a high level no color will develop.