Intended use of the testkit
The Leishmania SRE-kit is designed to detect antibodies against Leishmania. Leishmania antigen is bound to the well of the microtiter strip. After washing the well, “unknown” dog serum/plasma is added to the well. The antigen that is fixed to the well will capture antibodies to Leishmania if present in the sample. After incubation unbound material is washed away and the well is incubated with a specific HRPO conjugated antibody. After the last washing cycle substrate is added to detect bound antibodies, these will color blue if Leishmania antibodies are present in the sample.
Principle of the testkit
The test is based on the reaction of Leishmania proteins with dog antibodies. To this end Leishmania proteins have been coated to a 32-well microtiter plate.
The diluted dog serum/plasma sample is added to the wells of the coated plate. After washing the bound dog antibodies are detected by a HRPO conjugated anti-species conjugate. The color reaction in the wells is directly related to the concentration of Leismania antibodies in the serum/plasma sample.