Intended use of the test kit
The CCV ELISA test kit is designed to detect CCV antigens. CCV proteins are attached to the solid phase. After washing the strips are incubated with the dog faeces and polyclonal anti-CCV antibodies. The strips are washed after incubation to remove unbound materials. A HRPO labeled anti-species conjugate is added to detect bound polyclonal antibodies to CCV proteins. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.
Principle of the test kit
The test is based on the reaction of CCV antigen with polyclonal antibodies. To this end CCV proteins have been coated to a 96-well microtiter plate.
The dog sample is added (diluted 1:2) to the wells of the coated plate.
The dog sample also can be titrated using a 3-step dilution, starting with a dilution 1:2 (>1:6 >1:18 >1:54).
After washing the bound polyclonal antibodies are detected by a HRPO conjugated anti-species conjugate. The inhibition color reaction in the wells is directly related to the concentration of CCV antigen in the faeces sample.