Canine Corona Virus Antigen ELISA (96 wells)

 337,55

An ELISA test to detect Canine Corona Virus antigen in faeces samples.

SKU: D1005-AG01 Categories: ,

Description

Intended use of the test kit

The CCV ELISA test kit is designed to detect CCV antigens. CCV proteins are attached to the solid phase. After washing the strips are incubated with the dog faeces and polyclonal anti-CCV antibodies. The strips are washed after incubation to remove unbound materials. A HRPO labeled anti-species conjugate is added to detect bound polyclonal antibodies to CCV proteins. After incubation and rinsing, the substrate is added and the optical density is measured at 450 nm.

Principle of the test kit

The test is based on the reaction of CCV antigen with polyclonal antibodies. To this end CCV proteins have been coated to a 96-well microtiter plate.

Qualitative
The dog sample is added (diluted 1:2) to the wells of the coated plate.
Quantitative
The dog sample also can be titrated using a 3-step dilution, starting with a dilution 1:2 (>1:6 >1:18 >1:54).

After washing the bound polyclonal antibodies are detected by a HRPO conjugated anti-species conjugate. The inhibition color reaction in the wells is directly related to the concentration of CCV antigen in the faeces sample.

Protocol

Canine Corona virus ag

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