Intended use of the testkit
The Chlamydia SRE test kit is designed to detect antibodies against Chlamydia antigens. To this end Chlamydia antigens are attached to the solid phase. After washing, the plates are incubated with the dog sera to be tested. The plates are washed after incubation to remove unbound materials. A HRPO labeled anti-species conjugate is added to detect bound dog antibodies to Chlamydia antigen. After incubation and rinsing, the substrate is added and yhe optical density is measured at 620 nm or 450nm.
Principle of the testkit
The EVL SRE test detects antibodies against Chlamydia. To this end recombinant purified Chlamydia proteins are attached to the solid phase. After washing, the wells are incubated with the dog sera to be tested. After washing HRPO conjugate is added to detect the bound antibodies.