Intended use of the testkit
This diagnostic test system for BLV infected cattle is intended to identify BLV p24 gp51 antibodies in individual or pooled milk samples. The sensitivity of the test is adjusted to the standards of the European Community with reference to the E4 serum and meets the requirements for testing pooled milk samples in the European enzootic bovine leucosis (EBL) screening program. In contrast to test systems which make use of polyclonal antibodies or only one gp51 monoclonal antibody, this two monoclonal antibodies mediated ELISA gives a minimum of non-specific reactions.
Principle of the testkit
The principle of the test is based on the reaction of anti-BLV-gp51 antibodies in test samples with BLV antigen. After washing, test milk samples are added to the wells and, if present, anti-BLV-p24 and anti-BLV-gp51 antibodies from the samples will bind to the antigen. The complex is detected by a horseradish peroxidase (HRPO) conjugated monoclonal antibody directed against bovine IgG. Color reaction in the wells is directly related to the presence of BLV-p24 and BLV-gp51 antibodies in the sample.
Interpretation of test results
A sample is scored BLV negative if the OD value is below or equal to the average OD value of the negative control plus 0,150 OD units.
Negative: OD samples OD negative control plus 0,150 OD units.
Note: if an individual sample does not meet these criteria for being negative. The sample should be retested undiluted, 1:2 and 1:4 diluted.